About Us
Our Innovative Research
AAL works actively with quality researchers at top universities using quality ethical and research standards. At AAL we trust our ability to understand the science. We are excited by the future research possibilities now we have proven the molecular origin of pyrrole and the relationship to oxidative stress.
AAL’s director adapted and improved a basic test method to comply with Australian conditions and regulatory requirements. The pyrrole test was then introduced in Australia as a prerequisite for doctor training by the USA experts in 2004.
The core principals behind our research philosophy are:
Mental health is often associated with distinct biochemical profiles (and generally accompanies co-morbidities) which can be measured and managed.
AND.
If the body is given the fundamental materials it needs for self repair, it will have the capacity to do so.
Our research findings have validated of this philosophy.
Our research findings populate our lectures on urinary pyrroles and associated pathologies in the BBH Doctor training.
WHAT WE BELIEVE IN
Applied Analytical Laboratories is the world leading testing centre for pyrroles in urine. Established in 2009 with the purpose of providing reliable testing services and novel research in this area. AAL have been the first to distinguish, resolve, and report the pyrrole-pyrrolenone analyte concentrations as separate to the main interference compound (urobilinogen) in urine extracts. The quantitative determination of urobilinogen has also proven to be a very useful parameter. In addition, world class NMR (800MHz) techniques have been used to study the molecular mechanisms at play during the free radical oxidation of regulatory haem using model compounds. It is clear that urinary pyrroles (when correctly measured) are a powerful biomarker of oxidative stress.
This has led to further research projects in this area. Due to the scientific demonstration of the role of this waste product, clinical application has become more precise with improved patient outcomes.
Applied Analytical Laboratories Pty Ltd is a company incorporated according to law with a registered office and laboratory operating at 6/11 Logandowns Drive, Meadowbrook, QLD 4131. Brett Lambert is the Owner and Principal Scientist. Brett Lambert CV
The company also provides a variety of analytical and consulting services.
WHY CHOOSE US
-We distinguish pyrroles/pyrrolenones from urobilinogen
-Pyrrole = Oxidative stress
-Urobilinogen = bile flow / kidney function
-Our collection protocols prevent inhibit the active fraction from degrading
We are the only Laboratory in the world who have done the research to separate pyrrole from Urobilinogen in urine and give results for each. Our pyrrole figure is an accurate reflection of the severity of your oxidative stress. Urobilinogen tells you about your gut function and other complications. Our collection rules stop the fragile pyrroles from breaking down before the test so we get a reliable measure.
CLINICAL RESEARCH
We set up the Laboratory to be able to research with top Universities. Our work has changed this test from one with little credibility to a reliable repeatable pathology test which informs of pathology across mental health, haematology, gastroenterology, and urology. We continue to be active in world leading research.
PARTNERS AND COLLABORATION
Applied Analytical Laboratories and the organisations they are associated with, are committed to further research and development of robust, practical markers for aiding successful diagnosis and treatment of mental ill health, and to teaching the clinical application of this knowledge.
Please contact Brett Lambert at AAL if you wish to be involved in this research at any stage either as a researcher, a patient, or a health practitioner.
Research projects we have been involved with include:
Griffith University. 2013 to 2020
Adelaide University and Queen Elizabeth Hospital. 2010 and ongoing.
Centre of genomics and personalized health at Queensland University of Technology. 2020 to current.
Griffith Research Institute for Drug. Discovery Access to equipment clearance. 2015 and ongoing.
Evaluation of processes and extraction of compounds. AAL own facilities. Constant.
Since proving the science of pyrroles in urine there are a number of projects in discussion.
Publication of these projects.
Clinical application of the findings to date.
Brett Lambert MSc. Principal of Applied Analytical Laboratories has done extensive research in novel drug discoveries, vaccination research and animal and plant chemistry. As all his work has been confidential except for the papers produced listed here his work is not for public viewing. Brett worked with Astra Zeneca in the late 1990’s on novel drug discoveries. His skill with spectroscopic equipment (including – but not limited to) high field NMR and mass spectroscopy) is rare.
Brett’s understanding of the possible value of this test through his daily interactions with doctors and their patients over the last 15 years and his over 80,000 tests places him in a unique position to drive world leading research.
FUNDING
AAL work closely with BBH who initiate and support much of the research in which we participate. There is little funding available in this area so our progress is dependent on individuals and families, who have been helped by the doctors trained by BBH, to directly support us and seek funding for us. http://www.apanlabs.com/donate-to-research/
PARTNERS
AAL works closely with BBH and leading Universities. Brett Lambert Brett Lambert CV has access to the GRIDD as he has the skill to use some of the best research equipment in the Southern Hemisphere. Our drive is to prove the sciences at molecular levels.
TESTIMONIALS
BRETT LAMBERT CV
Who is BRETT LAMBERT MAppSc, the PROPRIETOR and CHIEF SCIENTIST, of APPLIED ANALYTICAL LABORATORIES?
/As proprietor of Applied Analytical Laboratories, Brett is determined to further understand the biochemical origin and role of excreted pyrroles (and related) in urine, and the molecular journey resulting in this biomarker of severity of oxidative stress. Fascinated by the relationship with mental health and how a reduction in pyrrole/pyrrolenones levels in urine improves patient outcomes, Brett drives research to find the why! His journey to this point has not been boring.
During year 11 & 12 chemistry, Mackay students were given the opportunity to learn laboratory techniques used in the sugar industry at the sugar school. It was here that Brett first met a major career influence – Dr Lionel Stock – who became his main mentor during the beginning of his chemistry undergraduate degree. Dr Stock’s PhD was supervised by Sir John Cornforth, the only Australian born Nobel Laureate in Chemistry (and Australian of the year, 1976).
After graduation Brett worked with Lionel at the Sugar School where he trained sugar chemists in analytical and inorganic chemistry. The Sugar School and Sugar Research Institute was populated with researchers with incredible skills.
Commencing his Post Graduate research in 1993, through the CSIRO Wendall Laboratory in Rockhampton, with access to organ baths, ELISA techniques & in-vitro facilities, he set off making and testing conjugates for vaccination of beef cattle against toxic Pimelea sp. plants. This work also included the synthesis, characterisation, functional group manipulations and in-vitro testing of complex molecules as well as antibodies. Free access to all manner of spectroscopic techniques made his post graduate research very rewarding. Supervised by Professor Graham Pegg (retired) Deputy Vice Chancellor (Research), Central Queensland University and Michael D’Occhio, currently Professor of Food Security at UQ – Gatton, being a stickler for a vigorous scientific process was ingrained.
Upon achieving his MAppSc, (and following a year with Queensland Dept of Natural Resources), Brett commenced work with the Astra Zeneca funded pharmaceutical research facility at Griffith University (currently known as The GRIDD). The project used high throughput screening of rainforest plant and Great Barrier Reef coral extracts to screen for pharmaceutical leads. Brett’s role was isolation and structural elucidation of the leads through use of many different types of chromatography as well as Nuclear Magnetic Resonance (NMR) and Mass Spectrometry (GC-MS and LC-MS).
Later, a consultancy led to Brett working at a Gold Coast based laboratory developing the Urinary pyrrole test in 2004. Testing of the USA Method showed distinct flaws and adaptations were made to make the test reliable and repeatable. The Pyrrole/pyrrolene-one is a waste product that is easily destroyed by heat and light. The current protocols of Applied Analytical Laboratory pyrrole sample collection, in Australia, are based on his development work.
Excited by the potential of this test in the understanding and management of mental health, in 2009 Brett set up an independent laboratory with standards acceptable to work with University research projects. Since 2010 AAL have been involved in all University based projects exploring these oxidative stress biomarkers, which has resulted in validation of the Urinary Pyrrole test with Dr Stephanie Fryar-Williams through strong control and patient cohort.
NOTE: No previous published work in the “Pyrrole” field had controls included to reference the pathological group.
AAL engaged NATA to commence an accreditation process. AAL complied with all the requirements above and beyond the expected standards. With changes to regulations during the accreditation period, TGA IVD classification and the compliance with laboratory standards of NATA, means AAL is able to conduct the pyrroles in urine test.
Since 2010, Brett has led work to establish pyrroles in urine as an irrefutable measure of oxidative stress, and correlated with symptoms, a measure of severity of mental health and the response to treatment. The resulting science has opened many exciting research projects now in the pipeline. Brett is also leading fundamental research into the understanding of the chemical mechanism of the expression of elevated urinary pyrroles initially by conducting spectroscopic studies on synthetic analogues of a model system. Dissemination of some of the findings to date are presented in the “Science of Pyrroles in Urine“, lecture in 2021. http://www.apanlabs.com/pyrrole-test/#pyrroles-in-urine-test
Profit from this lecture will fund further detailed biochemical research. As this is the only detailed research in this test in the world, this funding is incredibly important for improving the care for mental health issues, worldwide.
Bio Balance Health has created “The Science of Pyrroles in Urine” lecture in conjunction with Brett Lambert. The lecture has been released, to all health professionals within the BBH online education platform. BBH and AAL websites have registration details. https://biobalance.org.au/events/dr-training-program/
In summary: Brett Lambert MSc. Principal of Applied Analytical Laboratories has done extensive research in novel drug discoveries, vaccination research as well as animal and plant chemistry. As all his work has been confidential, except for the papers produced listed on the AAL website, his work is not for public viewing. Brett worked with Astra Zeneca in the late 1990’s on novel drug discoveries. His NMR and other spectroscopy skills have allowed him to produce research results not previously possible.
Brett’s understanding of the possible value of this test through his daily interactions with doctors and their patients over the last 17 years and his over 80,000 tests place him in a unique position to drive this world leading research.
OUR BELIEF
Mental health is a set of biochemical profiles which can be measured and managed.
If we give the body the fundamental materials, or components, that it needs to repair itself it will have the capacity to do so.