Pyrrole, Kryptopyrrole, Pyrrole Disorder, Pyrrole Condition, Pyrroluria, Kryptopyrole

These are all names applying to a chemical found in the urine which has been clinically shown to be associated with a number of chronic health issues. Please note the current correct term is High or Elevated Urinary Pyrroles. Research in Australia, not yet ready for publication, has shown Urinary Pyrroles is a Bio Marker of severity of oxidative stress.
 
No research has been conducted to show it is a condition, syndrome or disorder. It is not a diagnosis, but a measure of oxidative stress which must be managed for long term reduction of symptoms. Work has also shown, as Urinary Pyrroles are unstable, it is essential that collection and transportation of urine is done under strict standardized conditions to maximize reliability of results. Poor collection and handling can result in false readings.
 
What is important is the additional focus on both the urine measures and on identifying and managing the underlying oxidative stress. A doctor trained by Bio Balance Health will know how to do this and manage the process which is individualised to each patient. Exploration of a product in urine associated with mental health started with Abram Hoffer and Carl Pfeiffer (physician and biochemist) when they noted extracts of urine from schizophrenics turned mauve upon the addition of a specific reagent.
 
The identification of High Urinary Pyrroles has changed many lives as reduction of the pyrrole measure correlates with reduction of symptoms of many unexplained health issues. The understanding that very High Urinary Pyrrole is also often associated with some forms of mental ill health has reduced the stigma and the loss of hope felt by many with a mental health diagnosis. The processes to reduce high pyrroles are not meant to replace current chronic health management but to enhance it. Symptoms associated with high pyrrole vary as the underlying stress varies. This explains why there are so many symptoms and how each person with high pyrroles manifest different responses.
 
In 2018 Applied Analytical Laboratories became the first to include measurement of urobilinogen in their testing process. Urobilinogen is a by-product of normal gut function as opposed to oxidative stress and is co-measured in the assay. Separation of these urine components allows for identification of additional pathologies (e.g. obstructed bile duct, haemolytic anaemia, or hepatitis) and is critical for correct interpretation. The distinction allows a more accurate identification of the composition of the sample as falsely treated results can exacerbate symptoms and/or lead to vitamin B6 toxicity. The laboratory report will give clear reference ranges.
 
We recommend you use Bio Balance Health trained doctors who have experience in managing the process. Self-diagnosis and self-medication is fraught with difficulties and will not achieve optimal results. Applied Analytical Laboratories Pty Ltd (AAL) is the only dedicated Pyrrole testing laboratory in Australia. AAL are the leaders in investigating the origin of elevated urinary pyrroles.

 

Urine Collection Process for Pyrrole Analysis “Where do I go to get a Urine Pyrrole Test ?”

Get a Pyrrole Test Request Form

If your Doctor has provided you with an Applied Analytical Urinary Pyrrole Test request form, click to find your nearest collection agency.
If you are a new practitioner looking to refer a patient, please email to request a Urinary Pyrrole Test request form.

Cease Supplements

Patients must be naïve of zinc supplements or cease supplements 72 hours prior to tests if it’s the first time (to establish a base-line).
Do NOT cease Zinc if it is a follow-up test.

Note Medications

Medications used with Rheumatoid Arthritis and/and Urinary Tract Infections can interfere with the test, as does Rifampicin which changes the urine to a red/mauve colour. If you are taking these medications consult your physician as you will need to complete the course and/or abstain for a wash out period of 4 weeks prior to collecting a sample. For some, this is not possible.

Time Of Collection

Very high levels of urinary bilirubin DO also interfere. However this is rare and you will be informed if this is the case. The time of collection needs to be noted on the form in order to help account for obtained Urobilinogen levels.

Blood does NOT affect the test result, so spotting at the beginning or end of menstrual cycle will not interfere with the result. It is NOT recommended to collect during peak menstruation.

Use Applied Analytical Specimen Jar

Urine is to be collected in the supplied specimen jar and immediately covered with foil which protects it from light and heat and from incidental high energy electromagnetic radiation (EMR).

Flash Freeze

The sample is to be frozen immediately and kept covered and frozen from the time of collection until the time of testing in the laboratory.

Transport (Collection Agency)

Samples are transported in dry ice to keep the relative temperature below freezing (at -30oC). Your results will be sent back to your doctor.

Pyrrole Results very high or very low?

According to NPAAC guidelines, we will notify the practitioner when the urinary pyrrole result is greater than 400ug/dL, urobilinogen is greater than 16µmol/L, or other parameters are detected (such as, bilirubin, blood, ketones, protein, nitrites and leucocytes. These can illuminate if a patient has liver/kidney function issues, diabetes, infection or other protein dysregulation factors. Currently Applied Analytical Laboratories (AAL) is the only facility to meet this requirement and successfully participate in RCPA conducted proficiency programs for this system as an extra level of assurance.

As a result of our regulatory review and further research, what has changed is:

Accuracy

Improvement in the sensitivity of the testing process

Validated Reference Ranges

Reference ranges are now specific to adult Australians.

Distinction

Distinct separation of pyrrole and urobilinogen, and the reporting of both.

References

Biomark Res. 2015 Feb 6;3:3. doi: 10.1186/s40364-015-0028-1. eCollection 2015.
Biomarkers of a five-domain translational substrate for schizophrenia and schizoaffective psychosis. Fryar-Williams S1, Strobel JE.
Biomark Res. 2016 Nov 9;7:172. eCollection 2016.
Fundamental Role of Methylenetetrahydrofolate Reductase 677 C → T Genotype and Flavin Compounds in Biochemical Phenotypes for Schizophrenia and Schizoaffective Psychosis. Fryar-Williams S.
Front Psychiatry. 2016 Apr 14;7:48. doi: 10.3389/fpsyt.2016.00048. eCollection 2016.
Biomarker Case-Detection and Prediction with Potential for Functional Psychosis Screening: Development and Validation of a Model Related to Biochemistry, Sensory Neural Timing and End Organ Performance. Fryar-Williams S1, Strobel JE.
Clinical Psychological Science, 2016. Vol. 4(1), 144-162,
Nutritional interventions in clinical depression. Rechenberg, K. (2015)
Psychiatr. Pol 2014; 48(1): 75-88,
Does the usual dietary intake of patients with depression require vitamin mineral supplementation?
Stefańska E, Wendołowicz A, Kowzan U, Konarzewska B, Szulc A, Ostrowska L.
ACNEM Journal, Vol 29 No.3, 2010
“The Effectiveness of Targeted Nutrient Therapy In Treatment of Mental Illness – A Pilot Study”; Richard Stuckey, William Walsh, Brett Lambert.
Journal of Child & Adolescent Psychopharmacology, 2017 Nov; 27(9): 823-832;
Micronutrient Therapy for Violent and Aggressive Male Youth: An Open Label Trial; Hambly, J., Francis, K., Khan, S., Gibbons, K., Walsh, W., Lambert, B., Testa, C., & Haywood, A., doi: 10.1089/cap.2016.0199. Epub 2017 May 8.

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